(c) 2016 Justin Bois. This work is licensed under a Creative Commons Attribution License CC-BY 4.0. All code contained herein is licensed under an MIT license.
This tutorial exercise was generated from an Jupyter notebook. You can download the notebook here. You can also view it here. Use this downloaded Jupyter notebook to fill out your responses.
Describe in words the correlation coefficient between two images.
Discuss some of the dangers of "colocalization by eye." By this, I mean the typical activity of merging and green channel and a red channel, seeing yellow, and saying, "the molecules are colocalized."
Discuss the connection between computation of the probability of colocalization with the hacker stats techniques we learned in the seventh week of the class.
You read a paper and the authors report a Manders coefficient of 0.5. To assess this value, what other parameters of their image processing pipeline would you need to know?