{"cells": [{"cell_type": "markdown", "metadata": {}, "source": ["# Homework 4.2: Screening enzyme activity with a colorimetric assay (40 pts)\n", "\n", "**[Dataset download](https://s3.amazonaws.com/bebi103.caltech.edu/data/TrpB_screen.zip)**\n", "\n", "*This problem was primarily written by Patrick Almhjell, who also acquired the data set.*\n", "\n", ""]}, {"cell_type": "markdown", "metadata": {}, "source": ["## Directed evolution\n", "\n", "Directed evolution is an iterative approach to protein engineering that relies on the ability to develop a method for quantifying and comparing protein activity. This means that your starting protein (the \"parent\") must have some starting activity, and it must be detectable and reproducible during screening. The process looks this this:\n", "\n", "