Homework 6.1: Caulobacter growth part 2 (30 pts)¶
In problem 5.2, you analyzed images of single Caulobacter cells growing and dividing over time. Reread that problem statement to refresh yourself about the experiment.
If you completed that homework problem, use the segmentation results you stored in the CSV file for this problem. If you did not, you may download a CSV file here. If you got any sort of reasonable segmentation results, you should use your own results and not those I have provided.
We define a growth event as a time period during which a bacterium grows before dividing. After a division, a new growth event begins.
a) Annotate each frame of a bacterial growth movie with a growth event identifier. Add these identifiers to your data frame and store the result as a CSV file. We will come back to this again.
b) Make a plot where the bacterial areas are colored by the growth event label.
c) How to you expect the time between divisions to be distributed? Make ECDFs of the time between devisions for each bacterium. We will learn ways to compare them to theoretical distributions in coming lessons, but for now, comment on any empirical observations you have about the interdivision times.