Pairs bootstrap and correlation

Dataset download


[1]:
import numpy as np
import pandas as pd

import numba

import bebi103

import holoviews as hv
hv.extension('bokeh')

bebi103.hv.set_defaults()

We continue out analysis of the drone sperm quality data set. Let’s load it and remind ourselves of the content.

[2]:
df = pd.read_csv('../data/bee_sperm.csv', comment='#')
df.head()
[2]:
Specimen Treatment Environment TreatmentNCSS Sample ID Colony Cage Sample Sperm Volume per 500 ul Quantity ViabilityRaw (%) Quality Age (d) Infertil AliveSperm Quantity Millions Alive Sperm Millions Dead Sperm Millions
0 227 Control Cage 1 C2-1-1 2 1 1 2150000 2150000 96.7263814616756 96.726381 14 0 2079617 2.1500 2.079617 0.070383
1 228 Control Cage 1 C2-1-2 2 1 2 2287500 2287500 96.3498079760595 96.349808 14 0 2204001 2.2875 2.204001 0.083499
2 229 Control Cage 1 C2-1-3 2 1 3 87500 87500 98.75 98.750000 14 0 86406 0.0875 0.086406 0.001094
3 230 Control Cage 1 C2-1-4 2 1 4 1875000 1875000 93.2874208336941 93.287421 14 0 1749139 1.8750 1.749139 0.125861
4 231 Control Cage 1 C2-1-5 2 1 5 1587500 1587500 97.7925061050061 97.792506 14 0 1552456 1.5875 1.552456 0.035044

Correlation

We might wish to investigate how two measured quantities are correlated. For example, if the number of dead sperm and the number of alive sperm are closely correlated, this would mean that a given drone produces some quantity of sperm and some fraction tend to be dead. Let’s take a look at this.

[3]:
# Only use values greater than zero for log scale
inds = (df['Alive Sperm Millions'] > 0) & (df['Dead Sperm Millions'] > 0)

hv.Points(
    data=df.loc[inds, :],
    kdims=['Alive Sperm Millions', 'Dead Sperm Millions'],
    vdims=['Treatment']
).groupby(
    'Treatment'
).opts(
    logx=True,
    logy=True,
).overlay(
)
[3]:

There seems to be some correlation (on a log scale), but it is difficult to tell. We can compute the correlation with the Pearson correlation coefficient. It is the plug-in estimate of the correlation between variables (in this case alive and dead sperm). The correlation is the covariance divided by the geometric mean of the individual variances

The Pearson correlation coefficient is implemented with np.corrcoef(), but we will code our own. We will JIT it for speed.

[4]:
@numba.njit
def pearson_r(x, y):
    """
    Compute plug-in estimate for the Pearson correlation coefficient.
    """
    return (
        np.sum((x - np.mean(x)) * (y - np.mean(y)))
        / np.std(x)
        / np.std(y)
        / np.sqrt(len(x))
        / np.sqrt(len(y))
    )

We can use it to compute the Pearson correlation coefficient for the logarithm of alive and dead sperm.

[5]:
pearson_r(
    df.loc[inds, "Alive Sperm Millions"].values,
    df.loc[inds, "Dead Sperm Millions"].values,
)
[5]:
0.1785839325544448

Pairs bootstrap confidence intervals

How can we get a confidence interval on a correlation coefficient? We can again apply the bootstrap, but this time, the replicate is a pair of data, in this case a dead sperm count/alive sperm count pair. The process of drawing pairs of data points from an experiment and then computing bootstrap replicates from them is called pairs bootstrap. Let’s code it up for this example with the Pearson correlation.

Our strategy in coding up the pairs bootstrap is to draw bootstrap samples of the indices of measurement and use those indices to select the pairs.

[6]:
@numba.njit
def draw_bs_sample(data):
    """Draw a bootstrap sample from a 1D data set."""
    return np.random.choice(data, size=len(data))


@numba.njit
def draw_bs_pairs(x, y):
    """Draw a pairs bootstrap sample."""
    inds = np.arange(len(x))
    bs_inds = draw_bs_sample(inds)

    return x[bs_inds], y[bs_inds]

With our pairs sampling function in place, we can write a function to compute replicates.

[7]:
@numba.njit
def draw_bs_pairs_reps_pearson(x, y, size=1):
    """
    Draw bootstrap pairs replicates.
    """
    out = np.empty(size)
    for i in range(size):
        out[i] = pearson_r(*draw_bs_pairs(x, y))
    return out

Finally, we can put it all together to compute confidence intervals on the correlation. To start, we extract all of the relevant measurements as Numpy arrays to allow for faster resampling (and that’s what our Numba’d functions require).

[8]:
# Extract NumPy arrays (only use values greater than zero for logs)
inds = (df["Alive Sperm Millions"] > 0) & (df["Dead Sperm Millions"] > 0)

alive_ctrl = df.loc[
    (inds) & (df["Treatment"] == "Control"), "Alive Sperm Millions"
].values

alive_pest = df.loc[
    (inds) & (df["Treatment"] == "Pesticide"), "Alive Sperm Millions"
].values

dead_ctrl = df.loc[
    (inds) & (df["Treatment"] == "Control"), "Dead Sperm Millions"
].values

dead_pest = df.loc[
    (inds) & (df["Treatment"] == "Pesticide"), "Dead Sperm Millions"
].values

Now we can compute the bootstrap replicates using our draw_bs_pairs_reps_pearson() function.

[9]:
# Get reps
bs_reps_ctrl = draw_bs_pairs_reps_pearson(
    np.log(alive_ctrl), np.log(dead_ctrl), size=10000
)

bs_reps_pest = draw_bs_pairs_reps_pearson(
    np.log(alive_pest), np.log(dead_pest), size=10000
)

And from the replicates, we can compute and print the 95% confidence interval.

[10]:
# Get the confidence intervals
conf_int_ctrl = np.percentile(bs_reps_ctrl, [2.5, 97.5])
conf_int_pest = np.percentile(bs_reps_pest, [2.5, 97.5])

print(
    """
Correlation in control conf int:   [{0:.2f}, {1:.2f}]
Correlation in pesticide conf int: [{2:.2f}, {3:.2f}]
""".format(
        *tuple(conf_int_ctrl), *tuple(conf_int_pest)
    )
)

Correlation in control conf int:   [0.44, 0.70]
Correlation in pesticide conf int: [0.28, 0.67]

We see a clear correlation in both samples, with a wide, but positive, confidence interval. Note that we did this analysis on a log scale, since the data span several orders of magnitude.

Computing environment

[11]:
%load_ext watermark
%watermark -v -p numpy,pandas,numba,bokeh,holoviews,bebi103,jupyterlab
CPython 3.7.5
IPython 7.1.1

numpy 1.17.3
pandas 0.24.2
numba 0.46.0
bokeh 1.4.0
holoviews 1.12.6
bebi103 0.0.44
jupyterlab 1.1.4